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Polymerase Chain Reaction. Volume I. Alkami Quick Guide™ for PCR. A laboratory reference for the. Polymerase Chain Reaction. Description of Polymerase Chain Reaction with protocol, tips and FAQ. In addition to the template DNA and the Taq polymerase, PCR requires freePrimer design is a critical step in a PCR protocol. The set of primers should flank the fragment you intend to amplify from the DNA template. This chapter of the Protocols and Applications Guide provides protocols and background information about PCR and RT-PCR. Consult the user manual and troubleshooting guides to mitigate poor DNA quality. Ensure that no residual PCR inhibitors such as phenol, EDTA, and proteinase K PCR protocol, PCR steps · Thaw all reagents on ice. · Assemble reaction mix into 50 µL volume in a thin walled 0.2 mL PCR tubes. · Add reagents in following order: The Polymerase Chain Reaction (PCR) is a powerful and sensitive technique for DNA amplification (1). Taq DNA Polymerase is an enzyme widely used in PCR (2). The

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